Spain striker David Villa will miss Tuesday's World Cup qualifier against Liechtenstein after injuring a muscle during training.
Spain's team doctors had originally said Villa was doubtful after he pulled a muscle during Sunday's team practice. But the doctors ruled the 35-year-old striker unavailable hours later.
Villa played the final two minutes of Spain's 3-0 win over Italy on Saturday, his first appearance after a three-year absence from the national team.
Villa, who plays for New York City FC, is Spain's all-time leading scorer with 59 goals in 98 matches.