The Turkish Foreign Ministry summoned the Greek ambassador in Ankara Petros Mavroidis on Tuesday, according to diplomatic sources.
Sources said the ambassador was summoned to the ministry over recent remarks by Nikos Kotzias, who recently resigned as Greek foreign minister.
Kotzias has said that Greece was ready to extend its territorial waters from six miles to 12 miles in the Ionian Sea.
Foreign Minister Mevlüt Çavuşoğlu mentioned Greek intentions to expand their nautical border on Tuesday morning shortly before the ambassador was summoned.
On Thursday, the Turkish navy stopped a Greek frigate from harassing a Turkish research ship on the Turkish continental shelf off the coast of the Turkish Republic of Northern Cyprus. Ankara warned Athens after the incident to "abstain from acts that would cause an escalation in the region."
Turkey has consistently contested the Greek Cypriot administration's unilateral drilling activity in the Eastern Mediterranean, saying Turkish Cypriots also have rights to the resources around the area.