The Taliban are "trying to erase women from society in Afghanistan," British Foreign Secretary James Cleverly said, after international aid agencies were forced to pull out over a ban on female workers.
The Foreign Secretary urged the ruling Taliban regime to reverse the decision, warning that millions of Afghans would suffer as a result of the policy.
Meanwhile the United Nations' top official in Kabul met with a Taliban minister to discuss the decision and its consequences for humanitarian relief efforts.
The ban on women working for non-governmental organisations (NGOs) has already prompted some major international aid agencies to suspend operations in Afghanistan.
The Foreign Secretary said: "The Taliban are trying to erase women from society in Afghanistan.
"Banning women from working for NGOs will prevent millions of Afghans from accessing life-saving aid and supplies. This will impact everyone.
"The Taliban must urgently reverse this decision."
The measure – the latest restrictions on women's rights under the Taliban – was announced on Saturday by Qari Din Mohammed Hanif, the economy minister, allegedly because some female NGO employees in Afghanistan were not wearing headscarf, or hijab, correctly.
The UN mission in Afghanistan's acting head, Ramiz Alakbarov, met with Mr Hanif on Monday and called for a reversal of the ban.
Save the Children, the International Rescue Committee, the Norwegian Refugee Council and Care had been providing essential services and support amid plummeting living conditions before the ban.
David Miliband, the former foreign secretary who leads the International Rescue Committee, said its female staff were "a lifeline for millions of clients and we cannot work without them".
Christian Aid said it had paused work while it sought clarity over the announcement, and urged the Taliban to reverse the ban.
"Imposing a ban on female aid workers will only curtail our ability to help the growing number of people in need, and risks compounding the dire humanitarian crisis that women and girls face," Ray Hasan from Christian Aid said.
